14.14 Red cell folate (categories 3)

Red cell folate data available for STOP cohort only.

Over or below median (median level calculated in STOP) <14900 vs ≥14900.

14.14.1 Global tests

Type III ANOVA on main and interaction effects, and LM model summaries.

PRL (ng/ml)

	Chisq	Df	Pr(>Chisq)
(Intercept)	314.979852	1	<0.0001	***
rcfolate_gp3	2.701767	1	0.1	ns
GA_samp	91.008623	1	<0.0001	***

hPL (μg/ml)

	Chisq	Df	Pr(>Chisq)
(Intercept)	33.967504	1	<0.0001	***
rcfolate_gp3	2.501515	1	0.1	ns
GA_samp	1678.317810	1	<0.0001	***

GH2 (ng/ml)

	Chisq	Df	Pr(>Chisq)
(Intercept)	566.365724	1	<0.0001	***
rcfolate_gp3	4.710131	1	0.03	*
GA_samp	779.739710	1	<0.0001	***

Insulin (uU/ml)

model term	df1	df2	F.ratio	Chisq	p.value
rcfolate_gp3	1	Inf	1.930	1.930	0.2	ns
GA_samp	1	Inf	2.869	2.869	0.09	ns

HOMA-IR

model term	df1	df2	F.ratio	Chisq	p.value
rcfolate_gp3	1	Inf	1.630	1.630	0.2	ns
GA_samp	1	Inf	2.215	2.215	0.1	ns

Glucose (mmol/L)

model term	df1	df2	F.ratio	Chisq	p.value
rcfolate_gp3	1	Inf	2.315	2.315	0.1	ns
GA_samp	1	Inf	1.029	1.029	0.3	ns

14.14.2 Model summary

Ratio of Geometric means (95% CI)

PRL (ng/ml)

Var	Effect	P
(Intercept)	18.05 (13.12, 24.83)	<0.0001
rcfolate_gp3≥14900	1.07 (0.99, 1.16)	0.10
GA_samp	1.13 (1.10, 1.16)	<0.0001

hPL (μg/ml)

Var	Effect	P
(Intercept)	1.70 (1.42, 2.03)	<0.0001
rcfolate_gp3≥14900	0.96 (0.92, 1.01)	0.11
GA_samp	1.36 (1.34, 1.39)	<0.0001

GH2 (ng/ml)

Var	Effect	P
(Intercept)	0.06 (0.05, 0.07)	<0.0001
rcfolate_gp3≥14900	0.93 (0.88, 0.99)	0.03
GA_samp	1.33 (1.30, 1.36)	<0.0001

Insulin (uU/ml)

Var	Effect	P
(Intercept)	8.07 (5.51, 11.81)	<0.0001
rcfolate_gp3≥14900	1.08 (0.97, 1.20)	0.16
GA_samp	1.03 (1.00, 1.06)	0.09

HOMA-IR

Var	Effect	P
(Intercept)	1.74 (1.17, 2.61)	0.007
rcfolate_gp3≥14900	1.08 (0.96, 1.20)	0.20
GA_samp	1.03 (0.99, 1.06)	0.14

Glucose (mmol/L)

Var	Effect	P
(Intercept)	4.87 (4.56, 5.21)	<0.0001
rcfolate_gp3≥14900	1.02 (0.99, 1.04)	0.13
GA_samp	1.00 (0.99, 1.00)	0.31

14.14.3 Diagnostics

Model diagnositcs.

Diagnositcs for PRL (ng/ml)

Diagnositcs for hPL (μg/ml)

Diagnositcs for GH2 (ng/ml)

Diagnositcs for Insulin (uU/ml)

Diagnositcs for HOMA-IR

Diagnositcs for Glucose (mmol/L)

14.14.4 Estimated marginal means

Outcome	rcfolate_gp3	response	SE	df	lower.CL	upper.CL
PRL (ng/ml)	<14900	73.126	5.474	69.360	62.983	84.903
PRL (ng/ml)	≥14900	78.147	5.839	68.978	67.326	90.709
hPL (μg/ml)	<14900	59.412	1.867	67.936	55.800	63.258
hPL (μg/ml)	≥14900	57.204	1.797	67.858	53.728	60.904
GH2 (ng/ml)	<14900	1.495	0.051	100.542	1.397	1.600
GH2 (ng/ml)	≥14900	1.396	0.048	100.355	1.304	1.493
Insulin (uU/ml)	<14900	11.221	0.408	Inf	10.449	12.050
Insulin (uU/ml)	≥14900	12.091	0.474	Inf	11.196	13.057
HOMA-IR	<14900	2.371	0.092	Inf	2.198	2.558
HOMA-IR	≥14900	2.549	0.105	Inf	2.351	2.764
Glucose (mmol/L)	<14900	4.717	0.037	Inf	4.646	4.789
Glucose (mmol/L)	≥14900	4.802	0.043	Inf	4.720	4.886

14.14.5 Plots

14.14.6 Post-hoc comparisons

Outcome	Contrast	Ratio of Geometric means (95% CI)	P-value
PRL (ng/ml)	≥14900 / <14900	1.07 (0.99, 1.16)	0.1
hPL (μg/ml)	≥14900 / <14900	0.96 (0.92, 1.01)	0.1
GH2 (ng/ml)	≥14900 / <14900	0.93 (0.88, 0.99)	0.03
Insulin (uU/ml)	≥14900 / <14900	1.08 (0.97, 1.20)	0.2
HOMA-IR	≥14900 / <14900	1.08 (0.96, 1.20)	0.2
Glucose (mmol/L)	≥14900 / <14900	1.02 (0.99, 1.04)	0.1